LOCUS       pRSETA_EGFPA206K        3576 bp DNA     circular SYN 27-SEP-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 27, 2023 from SnapGene 7.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3576
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        19..37
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             68..90
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             98..859
                     /codon_start=1
                     /label=full ORF
                     /translation="MRGSHHHHHHGMASMVSKGEELFTGVVPILVELDGDVNGHKFSVS
                     GEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMP
                     EGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
                     NVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKL
                     SKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     CDS             110..127
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             131..142
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASM"
     CDS             143..859
                     /codon_start=1
                     /label=egfp
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     terminator      936..983
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1080..1535
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        1616..1720
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2752..3340
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 atctcgatcc cgcgaaatta atacgactca ctatagggag accacaacgg tttccctcta
       61 gaataatttt gtttaacttt aagaaggaga tatacatatg cggggttctc atcatcatca
      121 tcatcatggt atggctagca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
      181 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
      241 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
      301 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
      361 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
      421 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
      481 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
      541 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
      601 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
      661 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
      721 gctgctgccc gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga
      781 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
      841 ggacgagctg tacaagtaag aattcgaagc ttgatccggc tgctaacaaa gcccgaaagg
      901 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
      961 aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatct ggcgtaatag
     1021 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     1081 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     1141 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     1201 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     1261 agctttacgg cacctcgacc gcaaaaaact tgatttgggt gatggttcac gtagtgggcc
     1321 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     1381 actcttgttc caaactggaa caacactcaa ccctatcgcg gtctattctt ttgatttata
     1441 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaatatttaa
     1501 cgcgaatttt aacaaaatat taacgtttac aatttcgcct gatgcggtat tttctcctta
     1561 cgcatctgtg cggtatttca caccgcatac aggtggcact tttcggggaa atgtgcgcgg
     1621 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     1681 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     1741 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     1801 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     1861 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     1921 gagcactttt aaagttctgc tatgtgatac actattatcc cgtattgacg ccgggcaaga
     1981 gcaactcggt cgccccccgc ggtattctca gaatgacttg gttgagtact caccagtcac
     2041 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     2101 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     2161 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     2221 gaatgaagcc ataccaaacg acgagagtga caccacgatg cctgtagcaa tgccaacaac
     2281 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     2341 ctgaatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     2401 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     2461 ggggccagat ggtaagcgct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     2521 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     2581 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     2641 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     2701 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     2761 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     2821 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     2881 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     2941 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     3001 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3061 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     3121 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     3181 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     3241 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     3301 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     3361 tttacggttc ctgggctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     3421 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     3481 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     3541 gcctctcccc gcgcgttggc cgattcatta atgcag
//
