raw data (min-max) 1) Manually crop xy to 1250x1250 using ImageJ 2) Save 16bit tiff to 8-bit tiff using ImageJ min-max scaling 0-255 3) Use ImageJ file-> save as-> image sequence-> format: PNG name: img start at: 0 digits: 4 4) Run ffmeg command to convert image series to mp4: ffmpeg -y -r 50 -f image2 -i img%04d.png -movflags faststart -pix_fmt yuv420p -vcodec libx264 -vf scale=trunc(iw/2)*2:trunc(ih/2)*2 -preset veryslow -crf 18 figure.mp4 max z, raw data (min-max) 1) Manually crop xy to 1250x1250 using ImageJ 2) Use Image-> Stacks-> Z Project...-> Max Intensity (option) 3) Save 16bit tiff to 8-bit tiff using ImageJ min-max scaling 0-255 4) Use File-> Save As-> PNG max z, transformed (min-max) 1) Manually crop xy to 1250x1250 using ImageJ 2) Use Image-> Stacks-> Z Project...-> Max Intensity (option) 3) Use Image-> Scale: - X scake = 1 - Y scale = cosine(alpha) = cos(30) = 0.866 - Width = auto - Height = auto - Interpolation = Bilinear - Average when downsing = ticked - Create new window = ticked 4) Pad image to re-size to original using Image-> Adjust-> Canvas Size: - X = 1250 - Y = 1250 - Center - Zero fill = unticked 5) Save 16bit tiff to 8-bit tiff using ImageJ min-max scaling 0-255 6) Use File-> Save As-> PNG