max z, raw data (min-max) 1) No cropping applied 2) Use Image-> Stacks-> Z Project...-> Max Intensity (option) 3) Save 16bit tiff to 8-bit tiff using ImageJ min-max scaling 0-255 4) Use File-> Save As-> PNG max z, transformed (min-max) 1) No cropping applied 2) Use Image-> Stacks-> Z Project...-> Max Intensity (option) 3) Use Image-> Scale: - X scake = 1 - Y scale = cosine(alpha) = cos(30) = 0.866 - Width = auto - Height = auto - Interpolation = Bilinear - Average when downsing = ticked - Create new window = ticked 4) Pad image to re-size to original using Image-> Adjust-> Canvas Size: - X = 2060 - Y = 2048 - Center - Zero fill = unticked 5) Save 16bit tiff to 8-bit tiff using ImageJ min-max scaling 0-255 6) Use File-> Save As-> PNG max z, transformed (saturated) 1) No cropping applied 2) Use Image-> Stacks-> Z Project...-> Max Intensity (option) 3) Use Image-> Scale: - X scake = 1 - Y scale = cosine(alpha) = cos(30) = 0.866 - Width = auto - Height = auto - Interpolation = Bilinear - Average when downsing = ticked - Create new window = ticked 4) Pad image to re-size to original using Image-> Adjust-> Canvas Size: - X = 2060 - Y = 2048 - Center - Zero fill = unticked 5) Use Image-> Adjust-> Brightness/Contrast - Select 48-804 - Hit apply 6) Save 16bit tiff to 8-bit tiff using ImageJ min-max scaling 0-255 7) Use File-> Save As-> PNG