AMS-AGY (min-max)
1) Manually crop xy to 500x500 using ImageJ
2) Save 16bit tiff to 8-bit tiff using ImageJ min-max scaling 0-255
3) Select substack: slices 5-25
4) Use ImageJ file-> save as-> image sequence->
    format: PNG
    name: img
    start at: 0
    digits: 4
5) Use output directly

Nikon (min-max)
1) Manually crop xy to 500x500 using ImageJ
2) Save 16bit tiff to 8-bit tiff using ImageJ min-max scaling 0-255
3) Select substack: slices 5-25
4) Use ImageJ file-> save as-> image sequence->
    format: PNG
    name: img
    start at: 0
    digits: 4
5) Use output directly