AMS-AGY (min-max)
1) Manually crop xy to 1250x1250 using ImageJ
2) Save 16bit tiff to 8-bit tiff using ImageJ min-max scaling 0-255
3) Use File-> Save As-> PNG

Nikon (min-max)
1) Manually crop xy to 1250x1250 using ImageJ
2) Save 16bit tiff to 8-bit tiff using ImageJ min-max scaling 0-255
3) Use File-> Save As-> PNG