3D projection (min-max)
1) Manually crop xy to 500x500 using ImageJ
2) Save 16bit tiff to 8-bit tiff using ImageJ min-max scaling 0-255 
3) Update Image-> Properties with z pixel calibration:
    - calculate pixel size in xy: 6.5um/40x = 0.1625um
    - divide piezo step size by xy step: 0.5um/0.1625um = 3.08
    - set z pixel step size to 3.08
4) Use ImageJ Image-> Stacks-> 3D Project...->
    see screen shot for settings (1degree step, interpolate, default the rest)
5) Use ImageJ file-> save as-> image sequence->
    format: PNG
    name: img
    start at: 0
    digits: 4
6) Run ffmeg command to convert image series to mp4:
    ffmpeg -y -r 25 -f image2 -i img%04d.png -movflags faststart -pix_fmt yuv420p -vcodec libx264 -vf scale=trunc(iw/2)*2:trunc(ih/2)*2 -preset veryslow -crf 25 figure.mp4

3D projection, transformed (min-max)
1) Manually crop xy to 500x500 using ImageJ
2) Use Image-> Stacks-> Reslice...[/]: default settings
    - Output spacing = 1, Start at = top, everything else unticked
3) Go File-> New-> Script...
    - Select Langauge IJ1 Macro
    - Paste the following ***macro*** for image translation:
***
xTranslate=0;
yTranslate=0.1625;
/*Translate a stack of images so that the first image is moved n
pixels in the X direction, the second moved 2n pixels, the third 3n pixels
and so on..
the variable "n" is represented by xTranslate and yTranslate
*/
for(i=1;i<=nSlices;i++){
setSlice(i);
run("Translate...", "x="+i*xTranslate+" y="+i*yTranslate+" interpolation=Bilinear slice");
}
***
    - Adjust the 'yTranslate' value as needed according the alpha tilt of
    microscope and piezo step size:
        yTranslate = sin(alpha)/[z-piezo/(sCMOSpixel/mag)]
                   = sin(30) / [0.5/(6.5/40)] = 0.1625 (in this case)
    - Run macro on stack
4) Reverse the re-slice operation at step 3)
5) Use Image-> Scale...
    - X Scale = 1
    - Y Scale = cos(alpha) = cos(30) = 0.866
    - Z Scale = 1
    - Width, Height, Depth = 500, 500, 26
    - Interpolation = Bilinear
    - 3 boxes = ticked
    - Title = default...
6) Use Image-> Adjust-> Canvas Size...:
    - Width = 500
    - Height = 500 (i.e. pad image to orginal size for comparison)
    - Position = Center
    - Zero Fill = unticked
7) Save 16bit tiff to 8-bit tiff using ImageJ min-max scaling 0-255 
8) Update Image-> Properties with z pixel calibration:
    - calculate pixel size in xy: 6.5um/40x = 0.1625um
    - divide piezo step size by xy step: 0.5um/0.1625um = 3.08
    - set z pixel step size to 3.08
9) Use ImageJ Image-> Stacks-> 3D Project...->
    see screen shot for settings (1degree step, interpolate, default the rest)
10) Use ImageJ file-> save as-> image sequence->
    format: PNG
    name: img
    start at: 0
    digits: 4
11) Run ffmeg command to convert image series to mp4:
      ffmpeg -y -r 25 -f image2 -i img%04d.png -movflags faststart -pix_fmt yuv420p -vcodec libx264 -vf scale=trunc(iw/2)*2:trunc(ih/2)*2 -preset veryslow -crf 25 figure.mp4